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KMID : 0545120170270020380
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 2 p.380 ~ p.387
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Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line
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Hamid Faysal Bin
Kim Jin-Sun
Shin Cha-Gyun
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Abstract
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The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. However, the virus-host interaction, especially PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed that viral DNAs were mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is required for successful PFV replication, at least at/after the nuclear entry step of viral DNA. These findings highlight the obscure mysteries of PFV-host interaction and the requirement of TNPO3 for productive infection of PFV in 293T cells.
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KEYWORD
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Transportin-3, prototype foamy virus, 1-long terminal repeat, 293 T cell
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